• Product nameAnti-NSE2 antibody [OTI4A2]
    See all NSE2 primary antibodies
  • Description
    Mouse monoclonal [OTI4A2] to NSE2
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human NSE2 produced in E.coli (NP_775956).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY control and pCMV6-ENTRY NSE2, COS7 cells transiently transfected by pCMV6-ENTRY NSE2, HEK293T cells transfected with overexpress NSE2 plasmid
  • General notes

    Dilute in PBS (pH7.3) before use.

    The clone number has been updated from 4A2 to OTI4A2, both clone numbers name the same clone.



Our Abpromise guarantee covers the use of ab119074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 27 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.


  • FunctionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the NSE2 family.
    Contains 1 SP-RING-type zinc finger.
  • Post-translational
    Sumoylated, possibly via autosumoylation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C8orf36 antibody
    • E3 SUMO-protein ligase NSE2 antibody
    • hMMS21 antibody
    • hNSE2 antibody
    • methyl methanesulfonate sensitivity gene 21 antibody
    • MMS21 antibody
    • MMS21 homolog antibody
    • non-SMC element 2 antibody
    • Non-SMC element 2 homolog antibody
    • Non-structural maintenance of chromosomes element 2 homolog antibody
    • NSE2_HUMAN antibody
    • Nsmce2 antibody
    see all

Anti-NSE2 antibody [OTI4A2] images

  • All lanes : Anti-NSE2 antibody [OTI4A2] (ab119074) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY NSE2

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 27 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202447)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSE2 using ab119074 at a dilution of 1/100.
  • HEK293T cells transfected with either NSE2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119074 at a dilution of 1/100, and then analyzed by flow cytometry.

References for Anti-NSE2 antibody [OTI4A2] (ab119074)

ab119074 has not yet been referenced specifically in any publications.

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