• Product name
  • Description
    Rabbit polyclonal to NSF
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Rat NSF.

    (Peptide available as ab98286.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat brain; Rat hippocampus; Mouse brain; Mouse hippocampus as well as the following whole cell lysates: CHO/K1; PC12; NIH3T3.



Our Abpromise guarantee covers the use of ab87155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).


  • Function
    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
  • Sequence similarities
    Belongs to the AAA ATPase family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • N ethylmaleimide sensitive factor antibody
    • N ethylmaleimide sensitive factor like protein antibody
    • N ethylmaleimide sensitive fusion protein antibody
    • N-ethylmaleimide-sensitive fusion protein antibody
    • NEM sensitive fusion protein antibody
    • NEM-sensitive fusion protein antibody
    • NSF antibody
    • NSF_HUMAN antibody
    • SKD 2 antibody
    • SKD2 antibody
    • Vesicle fusing ATPase antibody
    • Vesicle-fusing ATPase antibody
    • Vesicular fusion protein NSF antibody
    • Vesicular-fusion protein NSF antibody
    see all

Anti-NSF antibody images

  • All lanes : Anti-NSF antibody (ab87155) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Hippocampus Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : CHO-K1 cell lysate Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 28 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab87155 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87155 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NSF antibody (ab87155)

This product has been referenced in:
  • Zhou Y  et al. N-ethylmaleimide-sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells. Mol Med Rep 14:1061-6 (2016). WB ; Human . Read more (PubMed: 27277949) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab87155.
Please use the links above to contact us or submit feedback about this product.


Sign up