Overview

  • Product name
  • Description
    Rabbit polyclonal to NSF
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Rat NSF.

    (Peptide available as ab98286.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat brain; Rat hippocampus; Mouse brain; Mouse hippocampus as well as the following whole cell lysates: CHO/K1; PC12; NIH3T3.

Properties

Applications

Our Abpromise guarantee covers the use of ab87155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

Target

  • Function
    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
  • Sequence similarities
    Belongs to the AAA ATPase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • N ethylmaleimide sensitive factor antibody
    • N ethylmaleimide sensitive factor like protein antibody
    • N ethylmaleimide sensitive fusion protein antibody
    • N-ethylmaleimide-sensitive fusion protein antibody
    • NEM sensitive fusion protein antibody
    • NEM-sensitive fusion protein antibody
    • NSF antibody
    • NSF_HUMAN antibody
    • SKD 2 antibody
    • SKD2 antibody
    • Vesicle fusing ATPase antibody
    • Vesicle-fusing ATPase antibody
    • Vesicular fusion protein NSF antibody
    • Vesicular-fusion protein NSF antibody
    see all

Images

  • All lanes : Anti-NSF antibody (ab87155) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Hippocampus Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : CHO-K1 cell lysate Whole Cell Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 28 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab87155 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87155 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Zhou Y  et al. N-ethylmaleimide-sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells. Mol Med Rep 14:1061-6 (2016). WB ; Human . Read more (PubMed: 27277949) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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