• Product nameAnti-NSFL1C antibody
  • Description
    Rabbit polyclonal to NSFL1C
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 21 and 272 of Human NSFL1C (NP_057227).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab95963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P 1/100 - 1/250.


  • FunctionReduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro).
  • Sequence similaritiesBelongs to the NSFL1C family.
    Contains 1 SEP domain.
    Contains 1 UBX domain.
  • Post-translational
    Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis.
  • Cellular localizationNucleus. Golgi apparatus > Golgi stack. Chromosome. Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks.
  • Information by UniProt
  • Database links
  • Alternative names
    • DJ776F14.1 antibody
    • MGC3347 antibody
    • NSF1C_HUMAN antibody
    • NSFL1 (p97) cofactor (p47) antibody
    • NSFL1 cofactor p47 antibody
    • NSFL1C antibody
    • OTTHUMP00000029972 antibody
    • OTTHUMP00000029974 antibody
    • p47 antibody
    • P47 protein antibody
    • p97 cofactor p47 antibody
    • SHP1 homolog antibody
    • UBX domain protein 2C antibody
    • UBX domain-containing protein 2C antibody
    • UBX1 antibody
    • UBXD10 antibody
    • UBXN2C antibody
    see all

Anti-NSFL1C antibody images

  • All lanes : Anti-NSFL1C antibody (ab95963) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 41 kDa
  • ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.

References for Anti-NSFL1C antibody (ab95963)

This product has been referenced in:
  • Davis EJ  et al. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 19:1093-100 (2012). WB ; Human . Read more (PubMed: 23042607) »
  • Mosbech A  et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol 19:1084-92 (2012). WB ; Human . Read more (PubMed: 23042605) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (4-12)
Sample Human Cell lysate - other (HEK 293)
Specification HEK 293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jul 30 2013