Overview

  • Product name
  • Description
    Rabbit polyclonal to NSL1
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human NSL1 (NP_056286.2)

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4833432M17Rik antibody
      • AI451597 antibody
      • C1orf48 antibody
      • DC31 antibody
      • DC8 antibody
      • DKFZp566O1646 antibody
      • Gm105 antibody
      • Gm984 antibody
      • Kinetochore associated protein NSL1 homolog antibody
      • Kinetochore-associated protein NSL1 homolog antibody
      • MIS14 antibody
      • NSL1 antibody
      • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
      • NSL1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NSL1 with ab70835 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
      Lane 1: ab70835 used for IP at 3 µg/mg lysate.
      Lane 2: immunoprecipitation with an other antibody
      Lane 3: ab70836 used for IP at 3 µg/mg lysate
      Lane 4: Control IgG IP
      Detection: Chemiluminescence with an exposure time of 3 minutes. For blotting ab70836 was used at 1 µg/ml.

    References

    ab70835 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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