Overview

  • Product nameAnti-NSL1 antibody
    See all NSL1 primary antibodies
  • Description
    Rabbit polyclonal to NSL1
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 230-281 of Human NSL1 (NP_056286.2)

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab70836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

Target

  • FunctionPart of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833432M17Rik antibody
    • AI451597 antibody
    • C1orf48 antibody
    • DC31 antibody
    • DC8 antibody
    • DKFZp566O1646 antibody
    • Gm105 antibody
    • Gm984 antibody
    • Kinetochore associated protein NSL1 homolog antibody
    • Kinetochore-associated protein NSL1 homolog antibody
    • MIS14 antibody
    • NSL1 antibody
    • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
    • NSL1_HUMAN antibody
    see all

Anti-NSL1 antibody images

  • Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
    Lane 1: ab70835 used for IP at 3 µg/mg lysate.
    Lane 2: immunoprecipitation with an other antibody
    Lane 3: ab70836 used for IP at 3 µg/mg lysate
    Lane 4: Control IgG IP
    For blotting ab70836 was used at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.
  • All lanes : Anti-NSL1 antibody (ab70836) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-NSL1 antibody (ab70836)

ab70836 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70836.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"