Overview

  • Product name
  • Description
    Rabbit polyclonal to NSL1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-245 of Human NSL1 (NP_056286).

  • Positive control
    • A431, H1299, HeLaS3, HepG2 whole cell lysate for WB. HeLa cells for IF.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833432M17Rik antibody
    • AI451597 antibody
    • C1orf48 antibody
    • DC31 antibody
    • DC8 antibody
    • DKFZp566O1646 antibody
    • Gm105 antibody
    • Gm984 antibody
    • Kinetochore associated protein NSL1 homolog antibody
    • Kinetochore-associated protein NSL1 homolog antibody
    • MIS14 antibody
    • NSL1 antibody
    • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
    • NSL1_HUMAN antibody
    see all

Images

  • Anti-NSL1 antibody (ab96728) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 32 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96728 at 1/200 dilution (image 1) and merged with DNA probe (image 2).

References

ab96728 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96728.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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