Overview

  • Product name
    Anti-NSL1 antibody
    See all NSL1 primary antibodies
  • Description
    Rabbit polyclonal to NSL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-245 of Human NSL1 (NP_056286).

  • Positive control
    • A431, H1299, HeLaS3, HepG2 whole cell lysate for WB. HeLa cells for IF.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
  • Information by UniProt
  • Database links
    • Alternative names
      • 4833432M17Rik antibody
      • AI451597 antibody
      • C1orf48 antibody
      • DC31 antibody
      • DC8 antibody
      • DKFZp566O1646 antibody
      • Gm105 antibody
      • Gm984 antibody
      • Kinetochore associated protein NSL1 homolog antibody
      • Kinetochore-associated protein NSL1 homolog antibody
      • MIS14 antibody
      • NSL1 antibody
      • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
      • NSL1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-NSL1 antibody (ab96728)
      Western blot - Anti-NSL1 antibody (ab96728)
      Anti-NSL1 antibody (ab96728) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 32 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-NSL1 antibody (ab96728)
      Immunocytochemistry/ Immunofluorescence - Anti-NSL1 antibody (ab96728)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96728 at 1/200 dilution (image 1) and merged with DNA probe (image 2).

    References

    ab96728 has not yet been referenced specifically in any publications.

    Publishing research using ab96728? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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