Anti-NSMAF antibody (ab81260)
Key features and details
- Rabbit polyclonal to NSMAF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NSMAF antibody
See all NSMAF primary antibodies -
Description
Rabbit polyclonal to NSMAF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from human NSMAF protein.
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Positive control
- Human brain lysate, RMS-13 rhabdosarcoma cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 104 kDa.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 104 kDa. |
Target
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Relevance
NSMAF is a novel adaptor protein which mediates the activation of sphingomyelinase by receptors such as tumor necrosis factor receptor type 1 and CD40 and subsequent apoptosis in cardiomyocytes. Inhibition of this factor may provide a novel intervention point for reducing ischemia/reperfusion injury. -
Database links
- Entrez Gene: 8439 Human
- Omim: 603043 Human
- SwissProt: Q92636 Human
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Alternative names
- Factor associated with N SMase activation antibody
- Factor associated with neutral sphingomyelinase antibody
- Factor associated with neutral sphingomyelinase activation antibody
see all
Images
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All lanes : Anti-NSMAF antibody (ab81260) at 1/400 dilution
Lane 1 : human
brain lysate
Lane 2 : RMS-13
rhabdosarcoma cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : mouse anti-rabbit HRP at 1/50000 dilution
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 5 minutes
Visualized using Pierce West Femto substrate system.
Datasheets and documents
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Datasheet download
References (1)
ab81260 has been referenced in 1 publication.
- Leidal AM et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol 22:187-199 (2020). PubMed: 31932738