Overview

  • Product nameAnti-NSMase2 antibody
    See all NSMase2 primary antibodies
  • Description
    Rabbit polyclonal to NSMase2
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human NSMase2.

    (Peptide available as ab95417.)

  • Positive control
    • This antibody gave a positive signal in Rat Brain tissue lysate and human lung tissue sections.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).

Target

  • FunctionCatalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
  • Tissue specificityPredominantly expressed in brain.
  • Sequence similaritiesBelongs to the neutral sphingomyelinase family.
  • Developmental stageUp-regulated during G0/G1 phases.
  • Cellular localizationGolgi apparatus membrane. Cell membrane. May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons. According to PubMed:15051724, it localizes to plasma membrane in confluent contact-inhibited cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cca1 antibody
    • Confluent 3Y1 cell-associated protein 1 antibody
    • Neutral sphingomyelinase 2 antibody
    • Neutral sphingomyelinase II antibody
    • NSMA2_HUMAN antibody
    • nSMase-2 antibody
    • nSMase2 antibody
    • SMPD3 antibody
    • Sphingomyelin phosphodiesterase 3 antibody
    • sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) antibody
    see all

Anti-NSMase2 antibody images

  • Anti-NSMase2 antibody (ab85017) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 102 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab85017 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85017, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
  • IHC image of ab85017 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-NSMase2 antibody (ab85017)

ab85017 has not yet been referenced specifically in any publications.

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