Overview

  • Product name
  • Description
    Mouse polyclonal to NSMCE1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Cow, Dog, Rhesus monkey, Orangutan
  • Immunogen

    Full length protein, corresponding to amino acids 1-256 of Human NSMCE1 (AAH18938.3).

  • Positive control
    • Lysate from 293T cells transfected with NSMCE1; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.
  • Cellular localization
    Nucleus. Chromosome, telomere
  • Database links
  • Alternative names
    • HSPC333 antibody
    • HSPC337 antibody
    • Non SMC element 1 homolog (S. cerevisiae) antibody
    • Non SMC element 1 homolog antibody
    • Non structural maintenance of chromosomes element 1 homolog antibody
    • NSE1 antibody
    • NSMCE 1 antibody
    see all

Images

  • All lanes : Anti-NSMCE1 antibody (ab168578) at 1 µg/ml

    Lane 1 : NSMCE1-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG

    Developed using the ECL technique.

    Predicted band size: 30 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling NSMCE1 using ab168578 at 10 µg/ml.

References

ab168578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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