Overview

  • Product name
  • Description
    Rabbit polyclonal to NSMCE1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: RGVTEDDGRP IYALVNLATT SISKMATDFA ENELDLFRKA LELIIDSETG, corresponding to amino acids 73-122 of Human NSMCE1

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately >36, 64 kDa (predicted molecular weight: 31 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double strand breaks.
  • Cellular localization
    Nucleus. Chromosome, telomere
  • Database links
  • Alternative names
    • HSPC333 antibody
    • HSPC337 antibody
    • Non SMC element 1 homolog (S. cerevisiae) antibody
    • Non SMC element 1 homolog antibody
    • Non structural maintenance of chromosomes element 1 homolog antibody
    • NSE1 antibody
    • NSMCE 1 antibody
    see all

Images

  • Anti-NSMCE1 antibody (ab66956) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 50,000 - 100,000 dilution

    Predicted band size : 31 kDa
    Observed band size : <36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

References

ab66956 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66956.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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