Anti-NSMCE4A antibody - C-terminal (ab178898)

Overview

  • Product name
    Anti-NSMCE4A antibody - C-terminal
    See all NSMCE4A primary antibodies
  • Description
    Rabbit polyclonal to NSMCE4A - C-terminal
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within Human NSMCE4A aa 240-289 (C terminal). The exact sequence is proprietary.
    Sequence:

    PVIQEERAMPAQLRRMEESHQEATEKEVERILGLLQTYFREDPDTPMSFF


    Database link: Q9NXX6

  • Positive control
    • Human fetal lung lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 98% PBS, 2% Sucrose
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling NSMCE4A with ab178898 at 1/250. Cells wew fixed with 4% PFA for 30 minutes and blocked with 5% goat serum + 1% BSA + 0.02% fish gelatin overnight at 4°C. Cells were incubated with the primary antibody overnight at 4°C. An Alexa Fluor 546-conjugated anti-rabbit IgG (1/500, room temperature, 2 hours) was used as the secondary antibody.

  • Anti-NSMCE4A antibody - C-terminal (ab178898) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size : 44 kDa

References

ab178898 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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