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Rabbit polyclonal to NSP 5 alpha 3 alpha
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 450 and 500 of human NSP 5 alpha 3 alpha (NP_001028725.1).
Whole cell lysate from HeLa cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab87801 was affinity purified using an epitope specific to NSP 5 alpha 3 alpha immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 119 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Highly expressed in testis. Barely detectable in other tissues. Also highly expressed in some cancer cell lines.
Involvement in disease
Note=A chromosomal aberration involving CYTSB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with PDGFRB.
Belongs to the cytospin-A family.
Contains 1 CH (calponin-homology) domain.
Information by UniProt
Cytokinesis and spindle organization B antibody
Cytospin B antibody
Western blot - Anti-NSP 5 alpha 3 alpha antibody (ab87801)
All lanes : Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-NSP 5 alpha 3 alpha antibody (ab87801)
Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"