Anti-NSP 5 alpha 3 alpha antibody (ab87801)

Overview

  • Product name
    Anti-NSP 5 alpha 3 alpha antibody
    See all NSP 5 alpha 3 alpha primary antibodies
  • Description
    Rabbit polyclonal to NSP 5 alpha 3 alpha
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 450 and 500 of human NSP 5 alpha 3 alpha (NP_001028725.1).

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab87801 was affinity purified using an epitope specific to NSP 5 alpha 3 alpha immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 119 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity
      Highly expressed in testis. Barely detectable in other tissues. Also highly expressed in some cancer cell lines.
    • Involvement in disease
      Note=A chromosomal aberration involving CYTSB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with PDGFRB.
    • Sequence similarities
      Belongs to the cytospin-A family.
      Contains 1 CH (calponin-homology) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytokinesis and spindle organization B antibody
      • Cytospin B antibody
      • Cytospin-B antibody
      • CYTSB antibody
      • CYTSB_HUMAN antibody
      • FLJ36955 antibody
      • HCMOGT 1 antibody
      • HCMOGT antibody
      • HCMOGT-1 antibody
      • NSP antibody
      • NSP5 antibody
      • Nuclear structure protein 5 antibody
      • Specc1 antibody
      • Spectrin domain with coiled coils 1 antibody
      • Sperm antigen HCMOGT 1 antibody
      • Sperm antigen HCMOGT-1 antibody
      • Sperm antigen with calponin homology and coiled coil domains 1 antibody
      • Sperm antigen with calponin homology and coiled-coil domains 1 antibody
      • sperm antigen with calponin-like and coiled coil domains 1 antibody
      • Structure protein NSP5a3a antibody
      • Structure protein NSP5a3b antibody
      • Structure protein NSP5b3a antibody
      • Structure protein NSP5b3b antibody
      see all

    Images

    • All lanes : Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 119 kDa
      Observed band size : 119 kDa


      Exposure time : 30 seconds
    • Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab87801 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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