Anti-NSP 5 alpha 3 alpha antibody (ab87801)
Key features and details
- Rabbit polyclonal to NSP 5 alpha 3 alpha
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NSP 5 alpha 3 alpha antibody -
Description
Rabbit polyclonal to NSP 5 alpha 3 alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region between residues 450 and 500 of human NSP 5 alpha 3 alpha (NP_001028725.1).
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Positive control
- Whole cell lysate from HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab87801 was affinity purified using an epitope specific to NSP 5 alpha 3 alpha immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 119 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 119 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Tissue specificity
Highly expressed in testis. Barely detectable in other tissues. Also highly expressed in some cancer cell lines. -
Involvement in disease
Note=A chromosomal aberration involving CYTSB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with PDGFRB. -
Sequence similarities
Belongs to the cytospin-A family.
Contains 1 CH (calponin-homology) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 92521 Human
- Omim: 608793 Human
- SwissProt: Q5M775 Human
- Unigene: 431045 Human
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Alternative names
- Cytokinesis and spindle organization B antibody
- Cytospin B antibody
- Cytospin-B antibody
see all
Images
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All lanes : Anti-NSP 5 alpha 3 alpha antibody (ab87801) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 119 kDa
Observed band size: 119 kDa
Exposure time: 30 seconds -
Detection of NSP 5 alpha 3 alpha in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87801 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab87801 has not yet been referenced specifically in any publications.