Overview

  • Product nameAnti-NSP A + NSP B antibody [RNL-4]
  • Description
    Mouse monoclonal [RNL-4] to NSP A + NSP B
  • SpecificityThis antibody reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative.
  • Tested applicationsFlow Cyt, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.

  • EpitopeThis antibody recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C).
  • General notesReticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.


    Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Primary antibody notes Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
  • Clonality Monoclonal
  • Clone numberRNL-4
  • MyelomaSp2/0-Ag14
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab8961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.
ICC 1/50 - 1/100.
IHC-Fr Use at an assay dependent dilution.
WB 1/100 - 1/500.

Target

  • RelevanceReticulon 1 (NSP) may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom.
  • Cellular localizationEndoplasmic reticulum membrane
  • Database links
  • Alternative names
    • Neuroendocrine specific protein antibody
    • NSP antibody
    • NSPA antibody
    • NSPB antibody
    • Reticulon A antibody
    • Reticulon B antibody
    • Reticulon1 antibody
    • RTN1 antibody
    • RTN1A antibody
    • RTN1B antibody
    see all

Anti-NSP A + NSP B antibody [RNL-4] images

  • Schematic representation of of the deduced domain structure of the three NSP-gene encoded proteins, NSP-A, NSP-B and NSP-C and mapping of the epitopes recognized by the different NSP monoclonal antibodies.

References for Anti-NSP A + NSP B antibody [RNL-4] (ab8961)

This product has been referenced in:
  • Tufi R  et al. Reduction of endoplasmic reticulum Ca2+ levels favors plasma membrane surface exposure of calreticulin. Cell Death Differ 15:274-82 (2008). Read more (PubMed: 18034188) »
  • Senden NH  et al. Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A. Eur J Cell Biol 69:197-213 (1996). Read more (PubMed: 8900485) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"