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This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
This antibody is a useful marker of yeast nuclear pores.
Our Abpromise guarantee covers the use of ab4641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 108,91 kDa (predicted molecular weight: 85 kDa).
1/10000 (cell lysates) - 1/50000 (nuclear fractions). For other (non-ECL) detection methods, 1/1000 - 1/5000.