Overview

  • Product name
    Anti-Nsr1p antibody [31C4]
  • Description
    Mouse monoclonal [31C4] to Nsr1p
  • Specificity
    This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.

Properties

Applications

Our Abpromise guarantee covers the use of ab4642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000. (yeast cells).
WB 1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000.

Target

  • Relevance
    Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • NSR1 antibody
      • Nuclear localization sequence binding protein antibody
      • p67 antibody
      • SHE5 antibody

    Images

    • All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution

      Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
      Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate

      Secondary
      Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution

      Performed under reducing conditions.

      Predicted band size : 45 kDa

      Image courtesy of an anonymous Abreview.

      See Abreview

    • ab4642 (1/5000) detecting Nsr1p in DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
      Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
      For further experimental details please refer to Abreview.

      See Abreview

    References

    ab4642 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells DDY1810)
    Specification
    Yeast cells DDY1810
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - methanol/acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (DDY1810)
    Loading amount
    0.5 µg
    Specification
    DDY1810
    Gel Running Conditions
    Reduced Denaturing (4-12% NUPage gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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