Overview

  • Product name
  • Description
    Rabbit polyclonal to NSUN2
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A synthetic peptide corresponding to a region of human NSUN2

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • Function
    RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.
  • Post-translational
    modifications
    Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 methycytoisine methyltransferase antibody
    • D13Wsu123e antibody
    • FLJ20303 antibody
    • hTrm4 antibody
    • MISU antibody
    • Myc induced SUN domain containing protein antibody
    • NOL1/NOP2/Sun domain family 2 antibody
    • NOL1/NOP2/Sun domain family 2 protein antibody
    • NOL1/NOP2/Sun domain family member 2 antibody
    • NSUN 2 antibody
    • NSUN2 antibody
    • NSUN2_HUMAN antibody
    • SAKI antibody
    • Substrate of AIM1/Aurora kinase B antibody
    • TRM4 antibody
    • tRNA (cytosine 5 ) methyltransferase antibody
    • tRNA (cytosine 5 ) methyltransferase NSUN2 antibody
    • tRNA (cytosine(34)-C(5))-methyltransferase antibody
    • tRNA (cytosine-5-)-methyltransferase antibody
    • tRNA methyltransferase 4 homolo antibody
    • tRNA methyltransferase 4 homolog antibody
    see all

Images

  • Anti-NSUN2 antibody (ab75493) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa

References

ab75493 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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