The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
FunctionMay have S-adenosyl-L-methionine-dependent methyl-transferase activity.
Tissue specificityUbiquitous. Detected in placenta, heart and skeletal muscle.
Involvement in diseaseNote=NSUN5 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the methyltransferase superfamily. RsmB/NOP family.
Post-translational modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR.