Overview

  • Product nameAnti-NT5C antibody
    See all NT5C primary antibodies
  • Description
    Mouse polyclonal to NT5C
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human NT5C protein

  • Positive control
    • HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionDephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP.
  • Tissue specificityDetected in skeletal muscle, heart and pancreas.
  • Sequence similaritiesBelongs to the 5'(3')-deoxyribonucleotidase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3''-pyrimidine nucleotidase antibody
    • 5' nucleotidase, deoxy (pyrimidine) antibody
    • 5' nucleotidase, deoxy (pyrimidine), cytosolic type C antibody
    • 5''(3'')-deoxyribonucleotidase antibody
    • 5'(3')-deoxyribonucleotidase, cytosolic type antibody
    • 5', 3'-nucleotidase, cytosolic antibody
    • 5-prime,3-prime-nucleotidase, cytosolic antibody
    • cdN antibody
    • Cytosolic 5'' antibody
    • cytosolic 5',3'-pyrimidine nucleotidase antibody
    • cytosolic type antibody
    • cytosolic type C,5',3'-nucleotidase antibody
    • cytosolic,uridine 5'-monophosphate phosphohydrolase 2 antibody
    • Deoxy 5' nucleotidase 1 antibody
    • Deoxy-5''-nucleotidase 1 antibody
    • Deoxyribonucleotidase, cytosolic, 1 antibody
    • DNT antibody
    • dNT-1 antibody
    • dNT1 antibody
    • Nt5c antibody
    • NT5C_HUMAN antibody
    • P5N2 antibody
    • PN-I antibody
    • PN-II antibody
    • Pyrimidine 5-prime nucleotidase 2 antibody
    • UMPH2 antibody
    • uridine 5'-monophosphate phosphohydrolase 2 antibody
    • uridine 5-prime monophosphate hydrolase 2 antibody
    see all

Anti-NT5C antibody images

  • Immunofluorescence of HeLa cells labelled with ab66977 at 10µg/ml.
  • All lanes : Anti-NT5C antibody (ab66977) at 1/500 dilution

    Lane 1 : NT5C transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate


    Predicted band size : 23 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)

References for Anti-NT5C antibody (ab66977)

ab66977 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"