Overview

  • Product nameAnti-NT5C1A antibody
    See all NT5C1A primary antibodies
  • Description
    Rabbit polyclonal to NT5C1A
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human NT5C1A.

  • Positive control
    • Extracts from MCF-7 cells and HepG2 cells. A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab75101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionDephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.
  • Tissue specificityHighly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.
  • Sequence similaritiesBelongs to the 5'-nucleotidase type 3 family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' nucleotidase cytosolic IA antibody
    • 5NT1A_HUMAN antibody
    • AMP specific 5' NT antibody
    • CN I antibody
    • CN IA antibody
    • cN-I antibody
    • cN-IA antibody
    • CN1 antibody
    • cN1A antibody
    • cNI antibody
    • cNIA antibody
    • Cytosolic 5' nucleotidase 1A antibody
    • Cytosolic 5' nucleotidase IA antibody
    • Cytosolic 5' nucleotidase type 1A antibody
    • Cytosolic 5''-nucleotidase 1A antibody
    • Cytosolic 5''-nucleotidase IA antibody
    • MGC119199 antibody
    • MGC119201 antibody
    • NT5C1A antibody
    • nucleotidase, 5-prime, cytosolic, IA antibody
    see all

Anti-NT5C1A antibody images

  • All lanes : Anti-NT5C1A antibody (ab75101) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from MCF-7 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of NT5C1A in A549 cells using ab75101, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-NT5C1A antibody (ab75101)

ab75101 has not yet been referenced specifically in any publications.

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