The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
FunctionMay have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
Sequence similaritiesBelongs to the 5'(3')-deoxyribonucleotidase family.
Western blot against tagged recombinant protein immunogen using ab56938 NT5C2 antibody at 1ug/ml. Predicted band size of immunogen is 88 kDa
References for Anti-NT5C2 antibody (ab56938)
This product has been referenced in:
Mitra AK et al. Genetic variants in cytosolic 5'-nucleotidase II are associated with its expression and cytarabine sensitivity in HapMap cell lines and in patients with acute myeloid leukemia. J Pharmacol Exp Ther339:9-23 (2011).
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