Anti-NT5C2 antibody (ab96084)
Key features and details
- Rabbit polyclonal to NT5C2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NT5C2 antibody -
Description
Rabbit polyclonal to NT5C2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish -
Immunogen
Recombinant fragment, containing a sequence corresponding to a region within amino acids 108 and 332 of Human NT5C2.
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Positive control
- HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. H1299 and HeLaS3 whole cell lysate, OVCA tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
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IHC-P |
1/100 - 1/250.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
IHC-P
1/100 - 1/250. |
Target
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Function
May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides. -
Sequence similarities
Belongs to the 5'(3')-deoxyribonucleotidase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281951 Cow
- Entrez Gene: 22978 Human
- Entrez Gene: 76952 Mouse
- Entrez Gene: 324845 Zebrafish
- Omim: 600417 Human
- SwissProt: O46411 Cow
- SwissProt: P49902 Human
- SwissProt: Q3V1L4 Mouse
see all -
Alternative names
- 5' nucleotidase (purine) cytosolic type B antibody
- 5NTC_HUMAN antibody
- cN II antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab96084 has been referenced in 4 publications.
- Fei X et al. TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I. Mol Ther Oncolytics 26:413-428 (2022). PubMed: 36159777
- Carlini F et al. Different Susceptibility of T and B Cells to Cladribine Depends On Their Levels of Deoxycytidine Kinase Activity Linked to Activation Status. J Neuroimmune Pharmacol N/A:N/A (2021). PubMed: 33851318
- Tzoneva G et al. Activating mutations in the NT5C2 nucleotidase gene drive chemotherapy resistance in relapsed ALL. Nat Med 19:368-71 (2013). WB . PubMed: 23377281
- Meyer JA et al. Relapse-specific mutations in NT5C2 in childhood acute lymphoblastic leukemia. Nat Genet 45:290-4 (2013). PubMed: 23377183