Overview

  • Product nameAnti-NTAL antibody
    See all NTAL primary antibodies
  • Description
    Rabbit polyclonal to NTAL
  • SpecificityThis polyclonal antibody reacts with NTAL, a 30 kDa transmembrane adaptor protein expressed in membrane microdomains (rafts) of B cells, NK cells and myeloid cells. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL

  • Positive control
    • RAMOS human lymphoma cell line, THP-1 human monocytic leukemia cell line. Negative control for WB: Jurkat human leukemia T-cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab36451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml (incubation 60 min at RT; or overnight at 4°C). Sample preparation: Lysis buffer with 1% laurylmaltoside. Use under non reducing conditions. Detects a band of approximately 30 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
    • Tissue specificityHighly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
    • Involvement in diseaseNote=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • Post-translational
      modifications
      Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
      May be polyubiquitinated.
    • Cellular localizationCell membrane. Present in lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • HSPC046 antibody
      • LAB antibody
      • LAT2 antibody
      • Linker for activation of B cells antibody
      • Linker for activation of B-cells antibody
      • Linker for activation of T cells family member 2 antibody
      • Linker for activation of T cells transmembrane adaptor 2 antibody
      • Linker for activation of T-cells family member 2 antibody
      • Membrane associated adapter molecule antibody
      • Membrane-associated adapter molecule antibody
      • Non T cell activation linker antibody
      • Non-T-cell activation linker antibody
      • NTAL_HUMAN antibody
      • WBSCR 5 antibody
      • Wbscr15 antibody
      • Williams Beuren syndrome chromosome region 15 antibody
      • Williams Beuren syndrome chromosome region 5 antibody
      • Williams-Beuren syndrome chromosomal region 15 protein antibody
      • Williams-Beuren syndrome chromosomal region 5 protein antibody
      • WSCR5 antibody
      see all

    Anti-NTAL antibody images

    • All lanes : Anti-NTAL antibody (ab36451) at 1 µg/ml

      Lane 1 : NTAL-transfected HEK-293 FT cells
      Lane 2 : Negative control: Jurkat human leukemia T-cell line

      Secondary
      Lane 2 : goat anti-rabbit antibody


      Performed under non-reducing conditions.

      Observed band size : 30 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 58 kDa (possible non-specific binding),65 kDa (possible non-specific binding),70 kDa (possible non-specific binding).

    References for Anti-NTAL antibody (ab36451)

    ab36451 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"