Anti-NTF97/Importin beta antibody (ab115882)


  • Product nameAnti-NTF97/Importin beta antibody
    See all NTF97/Importin beta primary antibodies
  • Description
    Goat polyclonal to NTF97/Importin beta
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide (C-


    ) corresponding to amino acids 851-863 from C terminus of human NTF97/Importin beta

  • Positive control
    • Daudi cell lysate (WB), Human Spleen tissue (IHC-P)



Our Abpromise guarantee covers the use of ab115882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionFunctions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • Sequence similaritiesBelongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • Cellular localizationCytoplasm. Nucleus envelope.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

Anti-NTF97/Importin beta antibody images

  • Anti-NTF97/Importin beta antibody (ab115882) at 0.03 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg
    developed using the ECL technique

    Predicted band size : 97 kDa
  • Human Spleen (formalin-fixed, paraffin-embedded) stained with ab115882 at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-NTF97/Importin beta antibody (ab115882)

ab115882 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I have run alignments of the immunogens used to create each of these products against drosophila Importin beta. Unfortunately, each immunogen had less than 70% homology with your target and most were less th...

Read More