Purification notesab70726 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Use at an assay dependent dilution.
FunctionHas both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Tissue specificityWidely expressed with highest levels in heart and lowest levels in lung and liver.
Sequence similaritiesBelongs to the Nth/MutY family.
Developmental stageExpression levels are regulated during the cell cycle with increased levels during early and mid S-phase.
Immunocytochemistry/ Immunofluorescence - Anti-NTH1 antibody [2660C1a] (ab70726)This image is courtesy of an anonymous Abreview
ab70726 staining NTH1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 45 minutes at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.