Overview

  • Product nameAnti-NTN3 antibody
    See all NTN3 primary antibodies
  • Description
    Rabbit polyclonal to NTN3
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 430-460 of Human NTN3 (Accessions: NP_006172.1).

  • Positive control
    • CEM cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab123726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 61 kDa.

Target

  • FunctionNetrins control guidance of CNS commissural axons and peripheral motor axons.
  • Tissue specificitySpinal cord.
  • Sequence similaritiesContains 3 laminin EGF-like domains.
    Contains 1 laminin N-terminal domain.
    Contains 1 NTR domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • NET3_HUMAN antibody
    • Netrin-2-like protein antibody
    • Netrin-3 antibody
    • NTN2L antibody
    • NTN3 antibody
    see all

Anti-NTN3 antibody images

  • ab123726 staining NTN3 in tumor xenograft in nude mouse with human breast cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 10 minutes at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 30 minutes at 37°C. An undiluted HRP polymer-conjugated goat anti-rabbit IgG monoclonal was used as the secondary antibody.

    See Abreview

  • Anti-NTN3 antibody (ab123726) at 1/100 dilution + CEM cell line lysates at 35 µg

    Predicted band size : 61 kDa

References for Anti-NTN3 antibody (ab123726)

ab123726 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Thermo -- Lab Vision Ultra V Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Trilogy™ Pretreatment Solution
Sample Human Tissue sections (Tumor xenograft in nude mouse with human breast ca)
Specification Tumor xenograft in nude mouse with human breast ca
Permeabilization No
Fixative Formaldehyde
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Submitted Jun 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"