Anti-Netrin-3 antibody (ab185200)
Key features and details
- Rabbit polyclonal to Netrin-3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Netrin-3 antibody -
Description
Rabbit polyclonal to Netrin-3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Netrin-3 aa 1-150.
Database link: O00634 -
Positive control
- Human plasma (IgG/HSA depleted)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa. |
Target
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Function
Netrins control guidance of CNS commissural axons and peripheral motor axons. -
Tissue specificity
Spinal cord. -
Sequence similarities
Contains 3 laminin EGF-like domains.
Contains 1 laminin N-terminal domain.
Contains 1 NTR domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4917 Human
- Omim: 602349 Human
- SwissProt: O00634 Human
- Unigene: 158336 Human
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Alternative names
- NET3_HUMAN antibody
- Netrin-2 antibody
- Netrin-2-like protein antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human oral mucosa tissue labeling Netrin-3 with ab185200 at 1/50 and counterstained with hematoxylin.
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All lanes : Anti-Netrin-3 antibody (ab185200) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251MG cell line lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Secondary
All lanes : Swine anti-rabbit HRP conjugated antibody at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 1 minute
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab185200 has been referenced in 2 publications.
- Jiang S et al. Targeting netrin-3 in small cell lung cancer and neuroblastoma. EMBO Mol Med 13:e12878 (2021). PubMed: 33719214
- Dominici C et al. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance. Nature 545:350-354 (2017). PubMed: 28445456