Overview

  • Product nameAnti-NUCKS1 antibody
    See all NUCKS1 primary antibodies
  • Description
    Rabbit polyclonal to NUCKS1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NUCKS1.

    (Peptide available as ab95415.)

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesThis antibody gave a positive result in ELISA against the immunizing peptide.
    Not yet tested in other applications.

    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-NUCKS1 antibody images

    • Anti-NUCKS1 antibody (ab77770) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 27 kDa
      Observed band size : 27 kDa
      Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes
    • IHC image of NUCKS1 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77770, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

    References for Anti-NUCKS1 antibody (ab77770)

    ab77770 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77770.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"