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ab112151 is designed to monitor cell apoptosis by measuring the apoptotic chromatin condensation. The compacted chromatin of apoptotic cells binds higher amounts of nuclear dye compared to the healthy cells. The fluorometric assay is based on the detection of the DNA contents in cells using our proprietary non-fluorescent dye that becomes strongly fluorescent upon binding to cellular DNA.
In normal cells, Nuclear Green DCS1 Dye is not cell permeable. However, in apoptotic cells, Nuclear Green DCS1 Dye can easily get into the cells with compromised plasma membranes. Once inside the cell, the dye binds to intracellular DNA producing highly fluorescent complexes which identify the cells as non-viable cells. The staining density with Nuclear Green DCS1 Dye can be measured with a flow cytometer at Ex/Em = 490/520 nm (FL1 channel) or a fluorescence microscope (FITC filter set).
ab112151 can be used together with our other apoptosis reagents, such as our ab112149 NIR Mitochondrial Membrane Potential Assay Kit, for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening apoptosis activators and inhibitors.
Visit our FAQs page for tips and troubleshooting.
Store ab112151 dessicated.
|Assay Buffer||1 x 100ml|
|Nuclear Green DCS1 Dye||1 x 500µl|
Our Abpromise guarantee covers the use of ab112151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
ab112151 has not yet been referenced specifically in any publications.