Anti-Nuclear Factor Erythroid 2 Related Factor 1 antibody (ab90524)

Overview

  • Product name
    Anti-Nuclear Factor Erythroid 2 Related Factor 1 antibody
    See all Nuclear Factor Erythroid 2 Related Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Nuclear Factor Erythroid 2 Related Factor 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide designed within residues: TYNMAPSALD SADLPPPSAL KKGSKEKQAD FLDKQMSRDE HRARAMKIPF, corresponding to internal amino acids 576 - 625 of Human Nuclear Factor Erythroid 2 Related Factor 1 (NP_003195).

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images



  • Predicted band size : 85 kDa
    Western blot analysis of HepG2 cell lysate labeling Nuclear Factor Erythroid 2 Related Factor 1 with ab90524 at 1.0µg/ml.
  • Anti-Nuclear Factor Erythroid 2 Related Factor 1 antibody (ab90524) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 85 kDa

References

ab90524 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please find the information below regarding the testing discount offer, to test ab90425 in ChIP. Also I have sent you ab90425 as a free of charge replacement for ab34682 which was mislabeled on our website. There sho...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Loading amount
10000 cells
Specification
A549 cells
Treatment
26 nM DMSO/bortezomib (BTZ) 24 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Oct 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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