Anti-Nuclear Factor Erythroid Derived 2 antibody (ab77321)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab77321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use at an assay dependent dilution. Detection limit is approximately 3ng/ml as a capture antibody when used against the immunogen.

Target

  • FunctionComponent of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
  • Tissue specificityExpressed in hematopoietic cells and also in colon and testis.
  • Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • DomainThe PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site.
  • Post-translational
    modifications
    Phosphorylated on serine residues (By similarity). In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
    Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
    Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation.
  • Cellular localizationNucleus > PML body. Cytoplasm. The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
  • Information by UniProt
  • Database links
  • Alternative names
    • erythroid-derived 2 45 kDa subunit antibody
    • Leucine zipper protein NF-E2 antibody
    • Leucine zipper protein NFE2 antibody
    • NF E2 antibody
    • nfe2 antibody
    • NFE2_HUMAN antibody
    • Nuclear factor (erythroid derived 2), 45kDa antibody
    • nuclear factor (erythroid-derived 2), 45kD antibody
    • Nuclear factor antibody
    • nuclear factor, erythroid 2 antibody
    • Nuclear factor, erythroid derived 2 45 kDa subunit antibody
    • p45 antibody
    • p45 NF-E2 antibody
    • p45 NFE2 antibody
    • Transcription factor NF-E2 45 kDa subunit antibody
    • Transcription factor NFE2 45 kDa subunit antibody
    see all

Anti-Nuclear Factor Erythroid Derived 2 antibody images

  • Anti-Nuclear Factor Erythroid Derived 2 antibody (ab77321) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Nuclear Factor Erythroid Derived 2 antibody (ab77321) at 5 µg/ml

    Lane 1 : Nuclear Factor Erythroid Derived 2 transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 41 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • ab77321 was used as a capture antibody and detection limit for recombinant tagged NFE2 is approximately 3ng/ml.

References for Anti-Nuclear Factor Erythroid Derived 2 antibody (ab77321)

ab77321 has not yet been referenced specifically in any publications.

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