Anti-Nuclear Matrix Protein p84 antibody (ab102684)

Overview

  • Product name
    Anti-Nuclear Matrix Protein p84 antibody
    See all Nuclear Matrix Protein p84 primary antibodies
  • Description
    Rabbit polyclonal to Nuclear Matrix Protein p84
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 469 and 628 of Human Nuclear Matrix Protein p84 (NP_005122).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates. NCIN87 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab102684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (PH8.0).

Target

  • Function
    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
  • Tissue specificity
    Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
  • Sequence similarities
    Contains 1 death domain.
  • Domain
    An intact death domain is needed for apoptosis.
  • Post-translational
    modifications
    Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
  • Cellular localization
    Cytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
  • Information by UniProt
  • Database links
  • Form
    Nuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
  • Alternative names
    • hTREX84 antibody
    • Death domain containing protein p84N5 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • hTREX84 antibody
    • Nuclear matrix protein p84 antibody
    • P84 antibody
    • p84N5 antibody
    • Tho 1 antibody
    • THO complex 1 antibody
    • THO complex subunit 1 antibody
    • Tho1 antibody
    • THOC 1 antibody
    • Thoc1 antibody
    • THOC1_HUMAN antibody
    see all

Anti-Nuclear Matrix Protein p84 antibody images

  • Anti-Nuclear Matrix Protein p84 antibody (ab102684) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 76 kDa
  • ab102684 at a 1/500 dilution, staining Nuclear Matrix Protein p84 in a paraffin-embedded NCIN87 xenograft.

References for Anti-Nuclear Matrix Protein p84 antibody (ab102684)

ab102684 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34)
Loading amount
10 µg
Specification
NSC34
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 (Microglia))
Loading amount
40 µg
Specification
BV2 (Microglia)
Treatment
10µg/ml LPS 4h
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mrs. Anke Jurisch

Verified customer

Submitted May 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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