Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)

Overview

  • Product nameAnti-Nuclear Matrix Protein p84 antibody [EPR5661]
    See all Nuclear Matrix Protein p84 primary antibodies
  • Description
    Rabbit monoclonal [EPR5661] to Nuclear Matrix Protein p84
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, U-87 MG and 293T cell lysates; Human stomach adenocarcinoma tissue; 293T cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab125019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
      Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
    • Tissue specificityUbiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
    • Sequence similaritiesContains 1 death domain.
    • DomainAn intact death domain is needed for apoptosis.
    • Post-translational
      modifications
      Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
    • Cellular localizationCytoplasm and Nucleus speckle. Nucleus > nucleoplasm. Nucleus matrix. Cytoplasm. Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.
    • Information by UniProt
    • Database links
    • FormNuclear (Isoform 1) and Cytoplasmic (Isoform 1 and 2).
    • Alternative names
      • hTREX84 antibody
      • Death domain containing protein p84N5 antibody
      • HPR 1 antibody
      • HPR1 antibody
      • hTREX84 antibody
      • Nuclear matrix protein p84 antibody
      • P84 antibody
      • p84N5 antibody
      • Tho 1 antibody
      • THO complex 1 antibody
      • THO complex subunit 1 antibody
      • Tho1 antibody
      • THOC 1 antibody
      • Thoc1 antibody
      • THOC1_HUMAN antibody
      see all

    Anti-Nuclear Matrix Protein p84 antibody [EPR5661] images

    • All lanes : Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : U-87 MG cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 76 kDa
      Observed band size : 84 kDa (why is the actual band size different from the predicted?)
    • ab125019, at 1/50 dilution, staining Nuclear Matrix Protein p84 in paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.
    • ab125019, at 1/100 dilution, staining Nuclear Matrix Protein p84 in 293T cells by Immunofluorescence.

    References for Anti-Nuclear Matrix Protein p84 antibody [EPR5661] (ab125019)

    ab125019 has not yet been referenced specifically in any publications.

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