Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477)

Overview

  • Product nameNuclear (Nucleic Acid-Free) Extraction Kit
  • Tests
    1 x 100 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.

    ab113477 can be used to extract nuclear proteins from mammalian cells and tissue samples free of nucleic acids attached.

    Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.

     

  • Notes

     

     

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113477 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Protocols

    References for Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477)

    ab113477 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your inquiry.

    We have two Nuclear Extraction Kits in our catalog at the moment:

    ab113474

    http://www.abcam.com/index.html?datasheet=113474 (or use the following: http://www.abcam.com/index.html?datasheet=11347...

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    Thank you for your inquiry.

    I am happy to confirm, that we do have several such kits.The classical kit for nuclear protein extraction is the ab113474. http://www.abcam.com/index.html?datasheet=113474 (or use the following: http://www.abcam.co...

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    Thank you for contacting Abcam regarding ab113477.

    This kit can be used to extract nucleic acid free nuclear proteins that can be used in protein-DNA binding assays.

    I hope this information is helpful. Please do not hesitate to co...

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    Thank you for contacting us.

    The lab sent the following advice:

    Yes, it would be sufficient to simply spin these down and begin with the protocol for suspension cells. However, the nuclear extractionwill be in the red color, which ...

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    Thank you for contacting us.

    The kit will be able to extract nuclear protein from intact nuclei of white blood cells but some percentage of the nuclei are likely to rupture when the samples are thawed. Have you tried thawing a sample to chec...

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    Thank you for your kind response. As I have explained in my previous e-mail any human lysate will probably have high levels of histone acetylation. However, it may be useful to treat the cells to maximize the level of acetylation. We have a very inform...

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    Thanks for your inquiry. 1. Regular nuclear extraction kit is better as nuclear proteins extracted with this kit can keep enzymes active compared to the kit for nucleic acid free extraction. We are not clear which demethylation assay is perf...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"