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Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
ab113477 can be used to extract nuclear proteins from mammalian cells and tissue samples free of nucleic acids attached.
Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.
|1000X DTT Solution||1 x 100µl|
|1000X Protease Inhibitor Cocktail (PIC)||1 x 100µl|
|10X Pre-Extraction Buffer||1 x 10ml|
|Extraction Buffer||1 x 10ml|
|Extraction Cleaner||1 x 100µl|
|Extraction Pre-Cleaner||1 x 1ml|
Our Abpromise guarantee covers the use of ab113477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113477 has not yet been referenced specifically in any publications.