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Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
ab113477 can be used to extract nuclear proteins from mammalian cells and tissue samples free of nucleic acids attached.
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|1000X DTT Solution||1 x 100µl|
|1000X Protease Inhibitor Cocktail (PIC)||1 x 100µl|
|10X Pre-Extraction Buffer||1 x 10ml|
|Extraction Buffer||1 x 10ml|
|Extraction Cleaner||1 x 100µl|
|Extraction Pre-Cleaner||1 x 1ml|
Our Abpromise guarantee covers the use of ab113477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113477 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"