The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesElectron Microscopy: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 106 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEssential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex.
Sequence similaritiesBelongs to the nucleoporin Nup84/Nup107 family.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
ab50008 (1:500, 1hr/ 37oC) staining Nuclear pore complex proteins in HeLa cells (green). Cells were fixed in PHEM buffer (45 mM Hepes pH 6.9, 45 mM Pipes pH 6.9, 5 mM MgCl2, 10 mM EGTA, 4% PFA) /0,2% Triton x-100, 10 min at room T°, then quickly wash 3 x in PBS. ) Good results are also obtained after straight PFA fix: fix in 3.7% PFA / 30 mM sucrose for 10 min at room T°, quickly wash 3 x in PBS, then incubatein PBS/ 0.1% TritonX-100 for 5 min and further in 0.1 M glycine for 10 min.Image kindly provided by R. Mangiacasale,M. Ciciarello and P. Lavia, Institute of. Molecular Biology and Pathology, CNR - Rome (Italy)
References for Anti-Nuclear Pore Complex Proteins antibody  (ab50008)
This product has been referenced in:
Light WH et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol11:e1001524 (2013).
Read more (PubMed: 23555195) »
Cubeñas-Potts C et al. SENP1 and SENP2 affect spatial and temporal control of sumoylation in mitosis. Mol Biol Cell24:3483-95 (2013).
Read more (PubMed: 24048451) »