Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008)

Overview

  • Product nameAnti-Nuclear Pore Complex Proteins antibody [414]
    See all Nuclear Pore Complex Proteins primary antibodies
  • Description
    Mouse monoclonal [414] to Nuclear Pore Complex Proteins
  • Specificityab50008recognizes several proteins in the nuclear pore complex.
  • Tested applicationsSuitable for: WB, IP, Electron Microscopy, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Saccharomyces cerevisiae, Xenopus laevis
  • Immunogen

    rat liver nuclei extract

  • Positive control
    • HeLa total cell extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone number414
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 106 kDa.
IP Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

Anti-Nuclear Pore Complex Proteins antibody [414] images

  • Lane 1 : Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008) at 1 µg/ml
    Lane 2 : Negative control - Goat Anti-Mouse IgG only

    Lane 1 : Extract from HeLa cells
    Lane 2 : As above

    Secondary
    Lane 1 : Goat Anti-Mouse IgG


    Predicted band size : 106 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • ab50008 (1:500, 1hr/ 37oC) staining Nuclear pore complex proteins in HeLa cells (green). Cells were fixed in PHEM buffer (45 mM Hepes pH 6.9, 45 mM Pipes pH 6.9, 5 mM MgCl2, 10 mM EGTA, 4% PFA) /0,2% Triton x-100, 10 min at room T°, then quickly wash 3 x in PBS. ) Good results are also obtained after straight PFA fix: fix in 3.7% PFA / 30 mM sucrose for 10 min at room T°, quickly wash 3 x in PBS, then incubatein PBS/ 0.1% TritonX-100 for 5 min and further in 0.1 M glycine for 10 min. Image kindly provided by R. Mangiacasale,M. Ciciarello and P. Lavia, Institute of. Molecular Biology and Pathology, CNR - Rome (Italy)

References for Anti-Nuclear Pore Complex Proteins antibody [414] (ab50008)

This product has been referenced in:
  • Eisch V  et al. Progerin impairs chromosome maintenance by depleting CENP-F from metaphase kinetochores in Hutchinson-Gilford progeria fibroblasts. Oncotarget 7:24700-18 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27015553) »
  • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »

See all 8 Publications for this product

Product Wall

I am very pleased to hear you would like to accept our offer and test one or both of these products in blocking experiements. Each code is associated with a paricular antibody so be sure tho reference the correct code when ordering and submitting the A...

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Thank you very much for your interest in our Nuclear Pore Complex Proteins antibodies. I have spoken to the labs about these product and have found that these are indeed the same clone detailed inin the papers by Davis & Blobel as we spoke of. Read More

Thank you for your enquiry. I can confirm this antibody is sourced externally. The originator has confirmed that it will recognise several proteins in the nuclear pore complex in various species. These proteins range in size between 62 and 210 kDa....

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Application Immunocytochemistry/ Immunofluorescence
Sample Kangaroo Cell (rat kangaroo kidney epithelial cell line PtK2)
Specification rat kangaroo kidney epithelial cell line PtK2
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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Siegfried Reipert

Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"