Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)

Overview

  • Product nameAnti-Nuclear Receptor Corepressor NCoR antibody
    See all Nuclear Receptor Corepressor NCoR primary antibodies
  • Description
    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • SpecificityThis antibody detects endogenous levels of total NCoR1 protein.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the internal sequence of Nuclear Receptor Corepressor NCoR (Human)

  • Positive control
    • MDA-MB-435 cell extract (Western blot). Human breast carcinoma tissue (IHC-P).

Applications

Our Abpromise guarantee covers the use of ab58396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
IHC-P
  • Application notesELISA: 1/20000.
    ICC/IF: Use at an assay dependent dilution (PMID 18311132).
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 270 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
    • Sequence similaritiesBelongs to the N-CoR nuclear receptor corepressors family.
      Contains 2 SANT domains.
    • DomainThe N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
      The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • hN CoR antibody
      • hNCoR antibody
      • KIAA1047 antibody
      • N CoR antibody
      • N Cor/SMRT corepressor Rip13 antibody
      • N CoR1 antibody
      • N-CoR antibody
      • N-CoR1 antibody
      • NCOR 1 antibody
      • NCoR antibody
      • Ncor1 antibody
      • NCOR1_HUMAN antibody
      • Nuclear receptor co repressor 1 antibody
      • Nuclear receptor corepressor 1 antibody
      • Retinoid X receptor interacting protein 13 antibody
      • RIP13 antibody
      • Rxrip13 antibody
      • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
      • TRAC 1 antibody
      • TRAC1 antibody
      see all

    Anti-Nuclear Receptor Corepressor NCoR antibody images

    • All lanes : Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396) at 1/500 dilution

      Lane 1 : MDA-MB-435 cell extract with no immunizing peptide
      Lane 2 : MDA-MB-435 cell extract with immunizing peptide


      Predicted band size : 270 kDa
    • ab58396, at 1/50 diltuion, staining human Nuclear Receptor Corepressor NCoR in breast carcinoma tissue by immunohistochemistry in the presence or absence of the immunizing peptide. Paraffin embedded tissue.

    References for Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396)

    This product has been referenced in:
    • Scaffidi P & Misteli T Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing. Nat Cell Biol 10:452-9 (2008). ICC/IF ; Human . Read more (PubMed: 18311132) »

    See 1 Publication for this product

    Product Wall

    Thank you for your reply.

    I am sorry the results you have observed have not improved with ab24552. From looking at the results you have shared you appear to be getting very similar results, with ab24552 showing a much clearer band at ˜250 kDa ...

    Read More

    Thank you for your reply and I am sorry for the delay in my response.

    In answer to your questions, I think the upper band you are seeing may well be the Nuclear Receptor Corepressor NCoR protein. It seems from your blot that there may be some ...

    Read More

    I am sorry for the delay in getting back to you.

    I now have confirmation of the protocol used with the Anti-Nuclear Receptor Corepressor NCoR antibody (ab58396). The Western blot presented on the datasheet of ab58396 was performed using 5% mil...

    Read More

    Thank you for your reply.

    Our experience is that some antibodies work much better under certain conditions. For example, the anti-GAPDH antibody ab9385 has been shown to work much more efficiently with BSA used in blocking than milk. This can ...

    Read More

    Thank you for your reply.

    I am sorry for the delay in my response. I will reply in English if you don't mind as I am more comfortable discussing scientific content in this language. By all means feel free to reply in Swedish, I will understand...

    Read More

    The western blot image on the datasheet for ab58396 shows that this antibody was tested on human MDA-MB-435 cells. We have not tested this antibody against rat samples in house, however, based on sequence homology this antibody can pick up a band betwe...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"