Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552)

Overview

  • Product nameAnti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade
    See all Nuclear Receptor Corepressor NCoR primary antibodies
  • Description
    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR - ChIP Grade
  • SpecificityThis antibody is specific to NCoR1,variant 1, Genbank Accession number AF044209, SwissProt ID O75376.
  • Tested applicationsSuitable for: ChIP, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 789-957 of Human Nuclear Receptor Corepressor NCoR

  • Positive control
    • HeLa nuclear lysate

Applications

Our Abpromise guarantee covers the use of ab24552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution.
IP Use at an assay dependent dilution. Use 2-3ul per IP (See references for protocol details).
WB 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionMediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • Sequence similaritiesBelongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • DomainThe N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hN CoR antibody
    • hNCoR antibody
    • KIAA1047 antibody
    • N CoR antibody
    • N Cor/SMRT corepressor Rip13 antibody
    • N CoR1 antibody
    • N-CoR antibody
    • N-CoR1 antibody
    • NCOR 1 antibody
    • NCoR antibody
    • Ncor1 antibody
    • NCOR1_HUMAN antibody
    • Nuclear receptor co repressor 1 antibody
    • Nuclear receptor corepressor 1 antibody
    • Retinoid X receptor interacting protein 13 antibody
    • RIP13 antibody
    • Rxrip13 antibody
    • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
    • TRAC 1 antibody
    • TRAC1 antibody
    see all

Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade images

  • Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552) at 1/1000 dilution + HeLa nuclear extract

    Predicted band size : 270 kDa
    Observed band size : 270 kDa

References for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552)

This product has been referenced in:
  • Bouttier M  et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . Read more (PubMed: 27604870) »
  • Heldring N  et al. NCoR controls glioblastoma tumor cell characteristics. Neuro Oncol 16:241-9 (2014). ChIP . Read more (PubMed: 24335696) »

See all 14 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 07 2014

Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - nuclear (MCF7)
Specification MCF7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 10%
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Submitted Dec 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - nuclear (mcf7)
Specification mcf7
Negative control IgG processed sample
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 10%
Positive control input of the nuclear lysate which has been processed without antibody and decrosslinked and precipitated DNA.
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Submitted Sep 05 2013

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Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Sep 05 2013

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - nuclear (MCF7)
Specification MCF7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
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Submitted Jun 07 2013

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF7)
Total protein in input 100 µg
Immuno-precipitation step Other - sepharose beads
Specification MCF7
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Submitted May 16 2013

Thank you for your reply.

I am sorry the results you have observed have not improved with ab24552. From looking at the results you have shared you appear to be getting very similar results, with ab24552 showing a much clearer band at ˜250 kDa ...

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Thank you for contacting Abcam earlier today.
As we discussed, I am sending you new aliquots of both ab24552 and ab26721 and your new order number is ***********.
Below are a few possible alternatives if the replacements are not successful: <...

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Vielen Dank für Ihre Anfrage - und für Ihre Geduld! Bitte entschuldigen Sie, dass die Antwort so lange auf sich warten ließ. Ich hatte das Labor, das den NCoR- Antikörper ab24552 herstellt, kontaktiert und musste sehr lange auf eine zufriedenstellen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"