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Overview
Product name
Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade
See all Nuclear Receptor Corepressor NCoR products (3) ...
Description
Rabbit polyclonal to Nuclear Receptor Corepressor NCoR - ChIP Grade
Specificity
This antibody is specific to NCoR1,variant 1, Genbank Accession number AF044209, SwissProt ID O75376.
Tested applications
ChIP, IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human, Monkey
Immunogen
Recombinant fragment, corresponding to amino acids 789-957 of Human Nuclear Receptor Corepressor NCoR
Positive control
HeLa nuclear lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Coactivators and repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution. (Use 2-3ul per IP (See references for protocol details).)
WB: 1/1000Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).
ICC/IF: Use at an assay dependent dilution.
Target
Function
Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.
Domain
The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
Post-translational
modifications
Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75376
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RIP13 antibody
- Rxrip13 antibody
- hCIT529I10 antibody
see all
Database links
- Entrez Gene: 9611 Human
- Omim: 600849 Human
- SwissProt: O75376 Human
- Unigene: 462323 Human
Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade images:
Western blot - Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552)
Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552) at 1/1000 dilution + HeLa nuclear extract
Predicted band size : 270 kDa
Observed band size : 270 kDa
References for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552)
This product has been referenced in:
- Thorne JLet al. Epigenetic control of a VDR-governed feed-forward loop that regulates p21(waf1/cip1) expression and function in non-malignant prostate cells. Nucleic Acids Res 39:2045-56 (2011). ChIP; Human.Read more (PubMed: 21088000) »
- Peng Het al. Transcriptional coactivator HCF-1 couples the histone chaperone Asf1b to HSV-1 DNA replication components. Proc Natl Acad Sci U S A 107:2461-6 (2010). ICC/IF; Monkey.Read more (PubMed: 20133788) »
See all 7 publications for this product
Publishing research using ab24552? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"