Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552)

Overview

  • Product name
    Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade
    See all Nuclear Receptor Corepressor NCoR primary antibodies
  • Description
    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR - ChIP Grade
  • Specificity
    This antibody is specific to NCoR1,variant 1, Genbank Accession number AF044209, SwissProt ID O75376.
  • Tested applications
    Suitable for: ChIP, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 789-957 of Human Nuclear Receptor Corepressor NCoR

  • Positive control
    • HeLa nuclear lysate

Applications

Our Abpromise guarantee covers the use of ab24552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution.
IP Use at an assay dependent dilution. Use 2-3ul per IP (See references for protocol details).
WB 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • Sequence similarities
    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • Domain
    The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hN CoR antibody
    • hNCoR antibody
    • KIAA1047 antibody
    • N CoR antibody
    • N Cor/SMRT corepressor Rip13 antibody
    • N CoR1 antibody
    • N-CoR antibody
    • N-CoR1 antibody
    • NCOR 1 antibody
    • NCoR antibody
    • Ncor1 antibody
    • NCOR1_HUMAN antibody
    • Nuclear receptor co repressor 1 antibody
    • Nuclear receptor corepressor 1 antibody
    • Retinoid X receptor interacting protein 13 antibody
    • RIP13 antibody
    • Rxrip13 antibody
    • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
    • TRAC 1 antibody
    • TRAC1 antibody
    see all

Images

  • Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552) at 1/1000 dilution + HeLa nuclear extract

    Predicted band size : 270 kDa
    Observed band size : 270 kDa

References

This product has been referenced in:
  • Chakraborty K  et al. The mito-DAMP cardiolipin blocks IL-10 production causing persistent inflammation during bacterial pneumonia. Nat Commun 8:13944 (2017). Read more (PubMed: 28074841) »
  • Viswakarma N  et al. Transcriptional regulation of mixed lineage kinase 3 by estrogen and its implication in ER-positive breast cancer pathogenesis. Oncotarget 8:33172-33184 (2017). ChIP . Read more (PubMed: 28388540) »

See all 26 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 07 2014

Application
ChIP
Detection step
Semiquantitative PCR
Sample
Human Cell lysate - nuclear (MCF7)
Specification
MCF7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 10%
Username

Abcam user community

Verified customer

Submitted Dec 10 2013

Thank you for your reply.

I am sorry the results you have observed have not improved with ab24552. From looking at the results you have shared you appear to be getting very similar results, with ab24552 showing a much clearer band at ˜250 kDa ...

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Thank you for contacting Abcam earlier today.
As we discussed, I am sending you new aliquots of both ab24552 and ab26721 and your new order number is ***********.
Below are a few possible alternatives if the replacements are not successful: <...

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Vielen Dank für Ihre Anfrage - und für Ihre Geduld! Bitte entschuldigen Sie, dass die Antwort so lange auf sich warten ließ. Ich hatte das Labor, das den NCoR- Antikörper ab24552 herstellt, kontaktiert und musste sehr lange auf eine zufriedenstellen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (vero)
Loading amount
20000 cells
Specification
vero
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (MCF-7 Breast Cancer cells)
Loading amount
25 µg
Specification
MCF-7 Breast Cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Corina Marx

Verified customer

Submitted Jul 20 2007

I have just received information from the source of the antibody saying "We normally use 2-3 ul of the N-CoR antibody for IP. We normally use HeLa nuclear extracts for IP, although it also worked for whole cell extracts from HeLa or 293T. I attach a co...

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Thank you for your enquiry. We qualify our "ChIP grade" antibodies in three ways: Through our; 1. In our in-house ChIP testing program, 2. In a scientific publication or 3. by a customer review. On this occasion this antibody was sourced fr...

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Thank you for your enquiry. We qualify our antibodies as "ChIP grade" status in three ways 1. in our in-house ChIP testing program, 2. in a scientific publication and 3. by a customer (Abreview). Rabbit polyclonal to NCoR2, ab24551 was qualifie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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