Anti-Nucleobindin 1 antibody (ab23360)

Overview

  • Product nameAnti-Nucleobindin 1 antibody
    See all Nucleobindin 1 primary antibodies
  • Description
    Rabbit polyclonal to Nucleobindin 1
  • Specificityab23360 recognises Nucleobindin 1
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: PAAHPEGQLK FHPDTDDVPV PAPAGDQKEV DTSEKKLLER LPEVEVPQHL conjugated to KLH, corresponding to a region within C terminal amino acids 413-462 of Human Nucleobindin 1

  • Positive control
    • HeLa cells, HepG2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMajor calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • Tissue specificityExpressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • Sequence similaritiesBelongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • DomainThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALNUC antibody
    • FLJ40471 antibody
    • NUC antibody
    • NUCB 1 antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin 1 antibody
    • Nucleobindin-1 antibody
    see all

Anti-Nucleobindin 1 antibody images

  • Golgi of HeLa cells are detected with ab23360 (green). Nuclei (red) were stained with DAPI.
  • Anti-Nucleobindin 1 antibody (ab23360) at 1 µg/ml + HepG2 lysates

    Predicted band size : 54 kDa

References for Anti-Nucleobindin 1 antibody (ab23360)

ab23360 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MDA-MB-231 breast cancer cell line)
Specification MDA-MB-231 breast cancer cell line
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.03%
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Oct 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"