Anti-Nucleobindin 1 antibody (ab55768)

Overview

  • Product nameAnti-Nucleobindin 1 antibody
    See all Nucleobindin 1 primary antibodies
  • Description
    Mouse monoclonal to Nucleobindin 1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-297 of Human Nucleobindin 1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMajor calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
    • Tissue specificityExpressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
    • Sequence similaritiesBelongs to the nucleobindin family.
      Contains 2 EF-hand domains.
    • DomainThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
    • Information by UniProt
    • Database links
    • Alternative names
      • CALNUC antibody
      • FLJ40471 antibody
      • NUC antibody
      • NUCB 1 antibody
      • NUCB1 antibody
      • NUCB1_HUMAN antibody
      • Nucleobindin 1 antibody
      • Nucleobindin-1 antibody
      see all

    Anti-Nucleobindin 1 antibody images

    • Nucleobindin 1 antibody (ab55768) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen tissue.


    • Predicted band size : 54 kDa
      Nucleobindin 1 antibody (ab55768) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

    References for Anti-Nucleobindin 1 antibody (ab55768)

    ab55768 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain slices)
    Specification Brain slices
    Fixative 4% paraformaldehyde + 0.2% picric acid
    Antigen retrieval step None
    Permeabilization Yes - Triton X-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

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    Submitted Aug 24 2012

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    DISCOUNT CODE (ab55768): XXXXXXXXXX

    Expiration date: 22-05-2012

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
    Loading amount 40 µg
    Specification MDA-MB-231 breast cancer cell line
    Gel Running Conditions Reduced Denaturing (gel 12 %)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Oct 15 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"