Anti-Nucleobindin 1 Precursor antibody [2A5] (ab119247)

Overview

  • Product nameAnti-Nucleobindin 1 Precursor antibody [2A5]
  • Description
    Mouse monoclonal [2A5] to Nucleobindin 1 Precursor
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human Nucleobindin 1 Precursor produced in HEK293T cells (NP_006175).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY Nucleobindin 1 Precursor, COS7 cells transiently transfected with Nucleobindin 1 Precursor
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 54 kDa.
ICC/IF 1/100.

Target

  • FunctionMajor calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • Tissue specificityExpressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • Sequence similaritiesBelongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • DomainThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationGolgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALNUC antibody
    • HGNC:8043 antibody
    • NUC antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin-1 antibody
    see all

Anti-Nucleobindin 1 Precursor antibody [2A5] images

  • All lanes : Anti-Nucleobindin 1 Precursor antibody [2A5] (ab119247) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Nucleobindin 1 Precursor cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 54 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201786)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleobindin 1 Precursor using ab119247 at a dilution of 1/100.

References for Anti-Nucleobindin 1 Precursor antibody [2A5] (ab119247)

ab119247 has not yet been referenced specifically in any publications.

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