Anti-Nucleolar protein 14 antibody (ab72741)

Overview

  • Product name
    Anti-Nucleolar protein 14 antibody
    See all Nucleolar protein 14 primary antibodies
  • Description
    Mouse polyclonal to Nucleolar protein 14
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human Nucleolar protein 14 (AAH26035).

  • Positive control
    • Human pancreas tissue lysate and Nucleolar protein 14 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects bands of approximately 89 and 105 kDa (predicted molecular weight for isoform 2: 91 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.
    • Sequence similarities
      Belongs to the NOP14 family.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C4orf9 antibody
      • chromosome 4 open reading frame 9 antibody
      • NOL14 antibody
      • NOP 14 antibody
      • NOP14 antibody
      • NOP14 nucleolar protein homolog (yeast) antibody
      • NOP14_HUMAN antibody
      • Nucleolar complex protein 14 antibody
      • Nucleolar protein 14 antibody
      • Nucleolar protein 14 homolog antibody
      • RES4 25 antibody
      • RES4-25 antibody
      • RES425 antibody
      • UTP2 antibody
      see all

    Images

    • Anti-Nucleolar protein 14 antibody (ab72741) at 1/500 dilution + human pancreas tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 91 kDa
      Observed band size : 89 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Nucleolar protein 14 antibody (ab72741) at 1/500 dilution

      Lane 1 : Nucleolar protein 14 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 91 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)

    References

    ab72741 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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