For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Nuclear matrix extract from MDCK cells (dog).
Our Abpromise guarantee covers the use of ab14966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent dilution. PubMed: 16571724|
|WB||1/1000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 85 kDa). A weak non-specific band of ~60kDa is detected in human cell lines with lower antibody dilutions.|
|IP||Use at an assay dependent dilution. (see Gotzmann et al reference).|
|ELISA||Use at an assay dependent dilution.|
|ICC||1/10 - 1/100.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"