Human nucleolin protein from Raji cell extract.
Our Abpromise guarantee covers the use of ab13541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa.|
|ICC||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use a concentration of 5 - 10 µg/ml.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ChIP||Use at an assay dependent concentration. PubMed: 19615975(recombinant nucleolin binding in the HPV16 genome).|
Immunocytochemistry/ Immunofluorescence analysis of 293 cells labeling Nucleolin with ab13541. Cells were fixed in chilled methanol for 30 min at −20°C, washed with PBS, and incubated in blocking solution (10% human serum in PBS) for 1 hour at room temperature. Cells were stained with Anti-Nucleolin antibody [4E2] - ChIP Grade (ab13541) diluted in blocking solution for 1 hour at room temperature in humidified chambers. Cells were washed with PBS, then incubated with secondary antibody diluted 1/200 in blocking solution for 1 hour at room temperature in opaque humidified chambers. Cells were washed with PBS, briefly rinsed in distilled H2O to remove salts, then mounted on glass slides.
ab13541 at 1µg/ml staining Nucleolin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody.
This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.