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Does not react withMouse
Human nucleolin protein from Raji cell extract.
Our Abpromise guarantee covers the use of ab13541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa.|
|ICC||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. Ab81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ChIP||Use at an assay dependent concentration. PubMed: 19615975(recombinant nucleolin binding in the HPV16 genome).|
ab 13541 at a 1/2000 dilution staining U2OS cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Alexa-Fluor ® 594 chicken anti-mouse antibody.
This image is courtesy of an Abreview submitted by Raffaela Santoro on 16 March 2006.
ab13541 at 1µg/ml staining Nucleolin in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"