Nuclear matrix extract from MDCK cells (dog).
Our Abpromise guarantee covers the use of ab14966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent dilution. PubMed: 16571724|
|WB||1/1000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 85 kDa). A weak non-specific band of ~60kDa is detected in human cell lines with lower antibody dilutions.|
|IP||Use at an assay dependent dilution. (see Gotzmann et al reference).|
|ELISA||Use at an assay dependent dilution.|
|ICC||1/10 - 1/100.|