- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Nucleolin antibody
See all Nucleolin primary antibodies -
DescriptionRabbit polyclonal to Nucleolin
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Nucleolin aa 435-463 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P19338 -
Positive control
- HepG2 cells, Lysate from 293 cells transfected with Nucleolin cDNA, Human breast tissue, HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesab170518 is purified through a protein A column, followed by peptide affinity purification
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/100 - 1/500. Predicted molecular weight: 77 kDa. | |
| ICC/IF | 1/10 - 1/50. |
Target
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FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
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Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
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Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Omim: 164035 Human
- SwissProt: P19338 Human
- Unigene: 79110 Human
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Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody (ab170518)Immunofluorescence analysis of HeLa cells, labeling Nucleolin using ab170518 at a 1/25 dilution. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody (ab170518)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast tissue, labeling Nucleolin using ab170518 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot - Anti-Nucleolin antibody (ab170518)All lanes : Anti-Nucleolin antibody (ab170518) at 1/100 dilution
Lane 1 : Lysate from nontransfected 293 cells
Lane 2 : Lysate from 293 cells transiently transfected with Nucleolin
Lysates/proteins at 2 µg per lane.
Predicted band size: 77 kDa -
Western blot - Anti-Nucleolin antibody (ab170518)Anti-Nucleolin antibody (ab170518) at 1/100 dilution + HepG2 cell lysate at 35 µg
Predicted band size: 77 kDa
Protocols
References
ab170518 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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