Overview

  • Product name
  • Description
    Rabbit polyclonal to Nucleolin
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Nucleolin aa 435-463 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P19338

  • Positive control
    • HepG2 cells, Lysate from 293 cells transfected with Nucleolin cDNA, Human breast tissue, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 77 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities
    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Images

  • Immunofluorescence analysis of HeLa cells, labeling Nucleolin using ab170518 at a 1/25 dilution. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast tissue, labeling Nucleolin using ab170518 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-Nucleolin antibody (ab170518) at 1/100 dilution

    Lane 1 : Lysate from nontransfected 293 cells
    Lane 2 : Lysate from 293 cells transiently transfected with Nucleolin

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 77 kDa
  • Anti-Nucleolin antibody (ab170518) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 77 kDa

References

ab170518 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170518.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up