The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
Use a concentration of 1 - 2 µg/ml.
1-2 µg/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.
Use a concentration of 10 - 20 µg/ml.
Use at an assay dependent concentration. PubMed: 20814900
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
Bender BJ et al. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol88:11738-47 (2014).
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