Overview

  • Product name
  • Description
    Rabbit polyclonal to Nucleolin
  • Tested applications
    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide:

    VKLAKAGKNQGDPKKMA

    conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Nucleolin with a C-terminal added cysteine.

  • Positive control
    • Whole extract of mouse NIH3T3 cells or rat PC12 cells, or human HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
IP Use a concentration of 1 - 2 µg/ml.

1-2 µg/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.

ICC/IF Use a concentration of 10 - 20 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 20814900

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities
    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Images

  • Indirect Immunofluorescence

    Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

References

This product has been referenced in:
  • García-Rubio ML  et al. The Fanconi Anemia Pathway Protects Genome Integrity from R-loops. PLoS Genet 11:e1005674 (2015). ICC/IF . Read more (PubMed: 26584049) »
  • Bender BJ  et al. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol 88:11738-47 (2014). Read more (PubMed: 25078694) »

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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