Synthetic peptide corresponding to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin
Our Abpromise guarantee covers the use of ab70493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 77 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|