Anti-Nucleolin (phospho T84) antibody (ab196338)

Overview

  • Product name
    Anti-Nucleolin (phospho T84) antibody
    See all Nucleolin primary antibodies
  • Description
    Rabbit polyclonal to Nucleolin (phospho T84)
  • Host species
    Rabbit
  • Specificity
    Reacts with Nucleolin when phoshosphorylated at threonine 84. Cross reacts with phoshothreonine 106 (pT106). Does not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of Phospho nucleolin can be done during mitosis or in G2 phase, there is no signal in G1S phase.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Nucleolin (phospho T84).
    Database link: P19338

Properties

Applications

Our Abpromise guarantee covers the use of ab196338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 77 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.

Target

  • Function
    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similarities
    Contains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

References

ab196338 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196338.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up