Anti-Nucleophosmin antibody (ab15440)
Key features and details
- Rabbit polyclonal to Nucleophosmin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Nucleophosmin antibody
See all Nucleophosmin primary antibodies -
Description
Rabbit polyclonal to Nucleophosmin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human Nucleophosmin aa 23-38.
Synthetic peptide corresponding to Human Nucleophosmin aa 226-240. -
Positive control
- WB: human HeLa, 293T, A431, A549, MCF7, U20S, K562, African green monkey COS7; mouse MEF, 3T3L1, C2C12; rat NRK. IHC-P: human kidney; ICC/IF: HeLa.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
|
ICC/IF | (1) |
1/100. Used at a dilution of 1/100 (see Abreview for further information).
|
Notes |
---|
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100. Used at a dilution of 1/100 (see Abreview for further information). |
Target
-
Function
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). -
Involvement in disease
A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. -
Sequence similarities
Belongs to the nucleoplasmin family. -
Post-translational
modificationsAcetylated at C-terminal lysine residues, thereby increasing affinity to histones.
ADP-ribosylated.
Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
Sumoylated by ARF. -
Cellular localization
Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis. - Information by UniProt
-
Database links
- Entrez Gene: 4869 Human
- Entrez Gene: 18148 Mouse
- Entrez Gene: 25498 Rat
- Omim: 164040 Human
- SwissProt: P06748 Human
- SwissProt: Q61937 Mouse
- SwissProt: P13084 Rat
- Unigene: 557550 Human
see all -
Alternative names
- B23 antibody
- MGC104254 antibody
- NO38 antibody
see all
Images
-
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa cells staining nucleophosmin with ab15440 at 1/100 dilution and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution (green). Nuclei were stained with ProLong™ Diamond Antifade Mountant with DAPI (blue); and F-actin was stained with Rhodamine Phalloidin (red). Negative control is using no primary antibody.
-
Immunohistochemical analysis of deparrafinized human kidney tissue staining nucleophosmin with ab15440 at 1/500 dilution, biotin-conjugated secondary antibody, SA-HRP and were counterstained with hematoxylin. Antigen retrieval method used was sodium citrate pH6
-
All lanes : Anti-Nucleophosmin antibody (ab15440) at 1/750 dilution
Lane 1 : Hela whole cell extract
Lane 2 : COS-7 whole cell extract
Lane 3 : U2OS whole cell extract
Lane 4 : HepG2 whole cell extract
Lane 5 : mouse brain tissue extract
Lane 6 : rat brain tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa -
All lanes : Anti-Nucleophosmin antibody (ab15440) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : MEF whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : U20S whole cell lysate
Lane 8 : K562 whole cell lysate
Lane 9 : COS7 whole cell lysate
Lane 10 : 3T3L1 whole cell lysate
Lane 11 : C2C12 whole cell lysate
Lane 12 : NRK whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-rabbit-HRP secondary antibody at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 32 kDaTris/Hcl gel used and proteins transferred to a PVDF membrane and blocked with 5% Milk/TBST.
Protocols
Datasheets and documents
-
Datasheet download
References (25)
ab15440 has been referenced in 25 publications.
- Beji S et al. Doxorubicin induces an alarmin-like TLR4-dependent autocrine/paracrine action of Nucleophosmin in human cardiac mesenchymal progenitor cells. BMC Biol 19:124 (2021). PubMed: 34134693
- Capella M et al. Nucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase. Nat Commun 12:4918 (2021). PubMed: 34389719
- Wang J et al. Triptolide interrupts rRNA synthesis and induces the RPL23-MDM2-p53 pathway to repress lung cancer cells. Oncol Rep 43:1863-1874 (2020). PubMed: 32236588
- Khaled SAA et al. Quantitative Assay of Mutated Nucleophosmin in Acute Myeloid Leukemia. J Hematol 8:111-120 (2019). PubMed: 32300454
- Malfatti MC et al. APE1 and NPM1 protect cancer cells from platinum compounds cytotoxicity and their expression pattern has a prognostic value in TNBC. J Exp Clin Cancer Res 38:309 (2019). PubMed: 31307523