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This product was produced with the following immunogens:
Synthetic peptide corresponding to Human Nucleophosmin aa 23-38.
Synthetic peptide corresponding to Human Nucleophosmin aa 226-240.
Our Abpromise guarantee covers the use of ab15440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100. Used at a dilution of 1/100 (see Abreview for further information).|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).|
Western blot analysis of various cell lines (25ug/lane) labeling Neucleophosmin with ab15440 at 1/500. A HRP-conjugated goat anti-rabbit IgG (1/20000) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Aparna Mitraab15440 staining human purified nucleophosmin from concentrated secreted serum free medium. 15 µg of purified protein were loaded in to each lane. The primary antibody was diluted 1/500 and incubated with the blot for 15 hours at 4°C. A HRP conjugated donkey anti-rabbit IgG antibody, diluted 1/5000, was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"